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. 1992 Jun 30;185(3):852-9.
doi: 10.1016/0006-291x(92)91705-u.

Activation of TIMP-2/progelatinase A complex by stromelysin

K Miyazaki  1 , F Umenishi, K Funahashi, N Koshikawa, H Yasumitsu, M Umeda
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Activation of TIMP-2/progelatinase A complex by stromelysin

K Miyazaki et al. Biochem Biophys Res Commun. .

Abstract

Progelatinase A was purified as a complex with TIMP-2 from the conditioned medium of a human glioblastoma cell line. The TIMP-2/progelatinase complex was resistant to the activation by p-aminophenylmercuric acetic acid (APMA), and showed less than 10% of the activity of the TIMP-2-free active enzyme. When the complex was incubated with stromelysin in the presence of APMA, the 64-kDa progelatinase was effectively converted to the 57-kDa mature enzyme, increasing its gelatinolytic activity about 8-fold. These results suggest that stromelysin is a natural activator of TIMP-2-bound progelatinase A.

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