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Comparative Study
. 1992 Jul;38(1):71-9.
doi: 10.1016/0166-0934(92)90170-i.

Comparison of several PCR procedures for detection of serum HCV-RNA using different regions of the HCV genome

I Castillo  1 , J Bartolomé, J A Quiroga, V Carreño
Affiliations
Comparative Study

Comparison of several PCR procedures for detection of serum HCV-RNA using different regions of the HCV genome

I Castillo et al. J Virol Methods. 1992 Jul.

Abstract

Different methods for the isolation of hepatitis C virus RNA and several sets of primers from the 5' untranslated, core, NS3, NS3/NS4 and NS5 regions of the hepatitis C virus genome were used for the detection of the viral genome by the polymerase chain reaction. Serum samples from 10 patients with chronic hepatitis C and 10 healthy controls were studied. The best method for the RNA extraction was with cold guanidinium isothiocyanate followed by a denaturation step prior to the polymerase chain reaction. Using this method, the percentage of positivity to hepatitis C virus sequences in serum depending on the region amplified were: 5' untranslated, 90%; Core, 20%; NS3, 80%; NS3/NS4, 60% and NS5, 60%.

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