doi: 10.1016/0378-4347(92)80183-q.
High-performance liquid chromatographic determination of rufloxacin and its main active metabolite in biological fluids
Affiliations
- PMID: 1323570
- DOI: 10.1016/0378-4347(92)80183-q
Item in Clipboard
High-performance liquid chromatographic determination of rufloxacin and its main active metabolite in biological fluids
J Chromatogr.
.
Abstract
A high-performance liquid chromatographic method is described for the determination of a fluoroquinolone, rufloxacin, and its N-desmethyl metabolite in plasma, urine and bile. Samples are chromatographed on a poly(styrene-divinylbenzene) column, the eluate being monitored with a fluorescence detector. The method was validated and a detection limit of 10 ng/ml for both rufloxacin and its metabolite in all the biological matrices considered was found. The method was successfully applied in pharmacokinetic studies.
Similar articles
-
Determination of the new fluoroquinolone balofloxacin and its metabolites in biological fluids by high performance liquid chromatography.Arzneimittelforschung. 1995 Jun;45(6):716-8. Arzneimittelforschung. 1995. PMID: 7646578
-
Analytical procedure for the determination of rufloxacin, a new pyridobenzothiazine, in human serum and urine by high-performance liquid chromatography.J Chromatogr. 1991 Mar 8;564(1):346-51. doi: 10.1016/0378-4347(91)80101-h. J Chromatogr. 1991. PMID: 1860932
-
Determination of BAY 12-8039, a new 8-methoxyquinolone, in human body fluids by high-performance liquid chromatography with fluorescence detection using on-column focusing.J Chromatogr B Biomed Sci Appl. 1997 Nov 21;702(1-2):163-74. doi: 10.1016/s0378-4347(97)00371-x. J Chromatogr B Biomed Sci Appl. 1997. PMID: 9449568 Clinical Trial.
-
Analysis of fluoroquinolones in biological fluids by high-performance liquid chromatography.J Chromatogr A. 1998 Jul 3;812(1-2):343-67. doi: 10.1016/s0021-9673(98)00274-x. J Chromatogr A. 1998. PMID: 9691331 Review.
-
Analysis of recent antimicrobial agents in human biological fluids by high-performance liquid chromatography.J Chromatogr A. 1998 Jul 31;815(2):163-72. doi: 10.1016/s0021-9673(98)00452-x. J Chromatogr A. 1998. PMID: 9718697 Review.
Cited by
-
Biliary excretion of rufloxacin in humans.Antimicrob Agents Chemother. 1993 Dec;37(12):2545-9. doi: 10.1128/AAC.37.12.2545. Antimicrob Agents Chemother. 1993. PMID: 8109915 Free PMC article.