Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Aug 25;20(16):4355-61.
doi: 10.1093/nar/20.16.4355.

Purification and characterization of a mammalian endo-exonuclease

C Couture  1 , T Y Chow
Affiliations
Free PMC article

Purification and characterization of a mammalian endo-exonuclease

C Couture et al. Nucleic Acids Res. .
Free PMC article

Abstract

An endo-exonuclease has been purified from cultured monkey (CV-1) cells. The enzyme which was purified to near homogeneity to be a 65 kDa monomeric protein. The single-strand DNase activity is endonucleolytic and nonprocessive, whereas the double-strand DNase activity is exonucleolytic and processive. The enzyme was also found to have RNase activity using poly-rA as substrate. The pH optimum for ss-DNase is 8 and for ds-DNase it is 7.5. Both DNase activities require a divalent metal ion (Mg2+, Mn2+, Ca2+, Zn2+) for activity and exhibit the same kinetics of heat inactivation. The purified protein binds to and cleaves a synthetic Holliday junction substrate. The overall enzymatic characteristics of the mammalian protein are very similar to the putative recombination endo-exonucleases purified from Neurospora crassa, Aspergillus nidulans and Saccharomyces cerevisiae.

PubMed Disclaimer

References

    1. Can J Biochem. 1978 Jun;56(6):370-7 - PubMed
    1. J Biol Chem. 1983 Oct 10;258(19):12010-8 - PubMed
    1. J Theor Biol. 1976 Jun;59(1):97-106 - PubMed
    1. Curr Genet. 1991 Jul;20(1-2):39-44 - PubMed
    1. Mol Cell Biol. 1991 Jun;11(6):3268-77 - PubMed

Publication types