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. 1992 Aug;58(8):2531-5.
doi: 10.1128/aem.58.8.2531-2535.1992.

Molecular cloning and mapping of phenol degradation genes from Bacillus stearothermophilus FDTP-3 and their expression in Escherichia coli

F M Dong  1 , L L Wang, C M Wang, J P Cheng, Z Q He, Z J Sheng, R Q Shen
Affiliations

Molecular cloning and mapping of phenol degradation genes from Bacillus stearothermophilus FDTP-3 and their expression in Escherichia coli

F M Dong et al. Appl Environ Microbiol. 1992 Aug.

Abstract

Two genes of the meta pathway of phenol degradation were cloned from a phenol-utilizing strain of Bacillus stearothermophilus and were mapped by subcloning and by use of a Tn5 insertion mutation. They code for phenol hydroxylase and catechol 2,3-dioxygenase, respectively. The gene encoding catechol 2,3-dioxygenase, which is more thermostable than catechol 2,3-dioxygenase encoded by the other gene, shares rather limited homology with that from Pseudomonas putida.

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