Flow cytometric analysis of nuclear DNA content of duct cell carcinoma of the pancreas
- PMID: 1325268
- DOI: 10.1002/1097-0142(19920901)70:5<1069::aid-cncr2820700510>3.0.co;2-u
Flow cytometric analysis of nuclear DNA content of duct cell carcinoma of the pancreas
Abstract
Background: This study was designed to evaluate the efficacy of nuclear DNA content analysis in determining the prognosis of carcinoma of the pancreas.
Methods: Resected and paraffin-embedded specimens from 72 patients with duct cell carcinoma of the pancreas were examined, and flow cytometry was used to explore the relationship between DNA ploidy and TNM classification or histologic grade.
Results: DNA aneuploidy was found histologically in 42.9%, 56.8%, and 71.4% of Grade 1, 2, and 3 tumors, respectively. DNA ploidy showed a statistically significant correlation with T category and retroperitoneal invasion. The cumulative survival rate of patients with retroperitoneal invasion was shorter than that of those without retroperitoneal invasion. Among the patients with retroperitoneal invasion, those with DNA aneuploidy had a significantly shorter survival time than did those with DNA diploidy.
Conclusions: The DNA ploidy pattern, in combination with the presence or absence of retroperitoneal invasion, appears to be useful in predicting the prognosis for duct cell adenocarcinoma of the pancreas.
Similar articles
-
Nuclear DNA content as a prognostic predictor in carcinoma of the pancreas.Int J Pancreatol. 1993 Aug;14(1):29-36. doi: 10.1007/BF02795227. Int J Pancreatol. 1993. PMID: 8409574
-
Heterogeneity in the nuclear DNA content of cells in carcinomas of the biliary tract and pancreas.Cancer. 1993 Nov 15;72(10):2920-8. doi: 10.1002/1097-0142(19931115)72:10<2920::aid-cncr2820721011>3.0.co;2-q. Cancer. 1993. PMID: 8221558
-
Prognostic significance of DNA ploidy in adenocarcinoma of the pancreas. A flow cytometric study of paraffin-embedded specimens.Cancer. 1993 Jun 15;71(12):3846-50. doi: 10.1002/1097-0142(19930615)71:12<3846::aid-cncr2820711209>3.0.co;2-i. Cancer. 1993. PMID: 8508352
-
Carcinoma in situ of the female breast. A clinico-pathological, immunohistological, and DNA ploidy study.APMIS Suppl. 2003;(108):1-67. APMIS Suppl. 2003. PMID: 12874968 Review.
-
Prognostic significance of morphological parameters and flow cytometric DNA analysis in carcinoma of the breast.Pathol Annu. 1990;25 Pt 1:171-210. Pathol Annu. 1990. PMID: 2153277 Review.
Cited by
-
A novel experimental mouse model of peritoneal dissemination of human gastric cancer cells: analysis of the mechanism of peritoneal dissemination using cDNA macroarrays.Jpn J Cancer Res. 2001 Jul;92(7):748-54. doi: 10.1111/j.1349-7006.2001.tb01157.x. Jpn J Cancer Res. 2001. PMID: 11473725 Free PMC article.
-
Expression of argyrophilic nucleolar organizer regions in ductal adenocarcinoma of the pancreas and its relationship to prognosis.Int J Pancreatol. 1993 Jun;13(3):193-200. doi: 10.1007/BF02924440. Int J Pancreatol. 1993. PMID: 8396611
-
Evaluation of DNA ploidy in relation with established prognostic factors in patients with locally advanced (unresectable) or metastatic pancreatic adenocarcinoma: a retrospective analysis.BMC Cancer. 2009 Jul 31;9:264. doi: 10.1186/1471-2407-9-264. BMC Cancer. 2009. PMID: 19646258 Free PMC article.
-
Heterogeneity of DNA ploidy pattern in carcinoma of the gallbladder: primary and metastatic sites.Jpn J Cancer Res. 1997 Sep;88(9):886-94. doi: 10.1111/j.1349-7006.1997.tb00465.x. Jpn J Cancer Res. 1997. PMID: 9369937 Free PMC article.
-
Establishment and characterization of a new transplantable pancreatic cancer xenograft (PZX-5) in immunosuppressed mice.Int J Pancreatol. 1998 Feb;23(1):51-62. doi: 10.1007/BF02787503. Int J Pancreatol. 1998. PMID: 9520091
Publication types
MeSH terms
Substances
LinkOut - more resources
Medical