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. 1992 Jun;37(2):149-50.
doi: 10.1007/BF01899737.

A rapid method for starting a culture for the establishment of Epstein-Barr virus-transformed human lymphoblastoid cell lines

Y Fukushima  1 , H Ohashi, K Wakui, T Nishida, T Oh-ishi
Affiliations

A rapid method for starting a culture for the establishment of Epstein-Barr virus-transformed human lymphoblastoid cell lines

Y Fukushima et al. Jpn J Hum Genet. 1992 Jun.

Abstract

We developed a simple and efficient procedure for the establishment of Epstein-Barr virus-transformed human lymphoblastoid cell lines. B-lymphocytes were obtained by centrifugation after hemolysis of red cells with a hemolysis buffer, instead of Ficoll-Parque gradient. We can start a primary culture within 15 min by using this method.

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