Preparation and characterization of a murine monoclonal antibody (MDR3M) reactive with mdr3 gene product
- PMID: 1328122
- PMCID: PMC5918957
- DOI: 10.1111/j.1349-7006.1992.tb01982.x
Preparation and characterization of a murine monoclonal antibody (MDR3M) reactive with mdr3 gene product
Abstract
A murine monoclonal antibody (MDR3M) (isotype: IgM) reactive with mdr3 gene product was generated by immunizing mice with mdr3-specific peptide (H2N-12WRPTSAEGDFELGISSKQKRKKTKTVKMI41G-COOH) and hybridizing the primed mouse splenic B cells with X63-Ag8,6.5.3 mouse plasmacytoma cells. MDR3M did not cross-react with mdr1 gene product. This monoclonal antibody may be useful for analyzing the role of mdr3 gene product in cells and tissues.
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