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Case Reports
. 1992 Aug;83(8):871-7.
doi: 10.1111/j.1349-7006.1992.tb01993.x.

New hepatocellular carcinoma cell line SUHC-1 established from a patient with hepatitis C virus RNA in serum

K Yoshizawa  1 , K Kiyosawa, S Usuda, K Yabu, Y Nakatsuji, S Yamada, K Furuta, E Tanaka, T Sodeyama, S Furuta, et al.
Affiliations
Case Reports

New hepatocellular carcinoma cell line SUHC-1 established from a patient with hepatitis C virus RNA in serum

K Yoshizawa et al. Jpn J Cancer Res. 1992 Aug.

Abstract

A new human hepatocellular carcinoma cell (HCC) line, designated SUHC-1, was derived from a Japanese patient with hepatocellular carcinoma having antibody to hepatitis C virus (HCV) and HCV-RNA in his serum, and established in tissue culture. This cell line exhibited typical epithelial cell morphology in culture as observed by phase-contrast and electron microscopy. The SUHC-1 cells produced albumin and alpha 2-macroglobulin. Chromosomal analysis showed several rearrangements at short and long arms of chromosome 1, 17 and 20 (1p-, 1q-, i(1q), i(17q) and 20q+) with a modal number of 91. HCV-RNA was not detected in the supernatant of SUHC-1 cells by nested polymerase chain reaction assay or in the SUHC-1 cells by the in situ hybridization method. We concluded that complete HCV does not exist in the SUHC-1 cell line. The SUHC-1 cell line is the first line of HCC to have been derived from a patient with persistent HCV infection, and may provide a suitable model for studies of hepatocarcinogenesis related to HCV.

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