Japanese encephalitis in Sri Lanka--the study of an epidemic: vector incrimination, porcine infection and human disease

Abstract

A prospective study of mosquito vectors, porcine infection and human disease was carried out during a Japanese encephalitis (JE) epidemic in the North Central province of Sri Lanka (November-December 1987) and a subsequent non-epidemic year (1988). The epidemic involved 361 cases of human encephalitis, virologically confirmed by immunoglobulin M enzyme-linked immunosorbent assay (ELISA), and was preceded 2-3 weeks earlier by sentinel porcine seroconversion. Virus isolation and viral antigen detection (ELISA) in field-caught mosquitoes incriminated Culex tritaeniorhynchus (Giles) and Cx gelidus Theobald as the major vectors of virus transmission during the porcine amplification and human 'spill-over' phases of the epidemic. Virus was also demonstrated in Cx fuscocephala Theobald, Cx whitmorei (Giles) and Mansonia uniformis (Theobald) during the epidemic. The major difference between the epidemic (1987) and non-epidemic (1988) years was a lower vector biomass and lower rates of virus carriage in the mosquito population.