[Transposition of Tn917 in Bacillus pumilus]
[Article in
Chinese]
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- PMID: 1329360
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[Transposition of Tn917 in Bacillus pumilus]
[Article in
Chinese]
Wei Sheng Wu Xue Bao.
1992 Aug.
Abstract
Transposition Tn917 was introduced into Bacillus pumilus 289 by protoplast transformation with plasmid pTV32. The temperature-sensitive replication property of pTV32 was maintained in B. pumilus. Tn917 was transposed efficiently in B. pumilus with 4.8 x 10(-4) transposition rate. The yield of auxotrophs was about 0.65% in all insertional mutants. It indicated a prospects for the use of Tn917 as a tool for insertional mutagenesis and genetic manipulation in B. pumilus.
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