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. 1992 Aug;32(4):305-7.

[Transposition of Tn917 in Bacillus pumilus]

[Article in Chinese]
Y Geng  1 , R Jiang
Affiliations
  • PMID: 1329360

[Transposition of Tn917 in Bacillus pumilus]

[Article in Chinese]
Y Geng et al. Wei Sheng Wu Xue Bao. 1992 Aug.

Abstract

Transposition Tn917 was introduced into Bacillus pumilus 289 by protoplast transformation with plasmid pTV32. The temperature-sensitive replication property of pTV32 was maintained in B. pumilus. Tn917 was transposed efficiently in B. pumilus with 4.8 x 10(-4) transposition rate. The yield of auxotrophs was about 0.65% in all insertional mutants. It indicated a prospects for the use of Tn917 as a tool for insertional mutagenesis and genetic manipulation in B. pumilus.

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