doi: 10.1016/1046-5928(92)90060-a.
Heterologous expression of recombinant human glutathione transferase A1-1 from a hepatoma cell line
Affiliations
- PMID: 1330133
- DOI: 10.1016/1046-5928(92)90060-a
Item in Clipboard
Heterologous expression of recombinant human glutathione transferase A1-1 from a hepatoma cell line
Protein Expr Purif.
1992 Feb.
Abstract
A cDNA clone, lambda GTHA1, encoding human glutathione transferase A1-1 has been isolated from a hepatoma HepG2 cDNA library. At the nucleotide level, the new clone showed minor differences from cDNA deriving from normal liver, but the deduced amino acid sequence was identical to the structure previously described. The protein was expressed from a plasmid, pKHA1, and isolated by a single-step affinity purification on an S-hexylglutathione Sepharose matrix. The yield of the recombinant protein was 165 mg from a 3-liter culture of bacteria.
Similar articles
-
Partial purification and characterization of the soluble glutathione transferase isoenzymes from cultured Hep G2 cells.Cell Biol Int Rep. 1989 Jul;13(7):585-93. doi: 10.1016/0309-1651(89)90108-2. Cell Biol Int Rep. 1989. PMID: 2551510
-
High-level bacterial expression of human glutathione transferase P1-1 encoded by semisynthetic DNA.Protein Expr Purif. 1995 Jun;6(3):265-71. doi: 10.1006/prep.1995.1034. Protein Expr Purif. 1995. PMID: 7663160
-
Characterization of S-hexylglutathione-binding proteins of human hepatocellular carcinoma: separation of enoyl-CoA isomerase from an Alpha class glutathione transferase form.Biochem J. 1997 Dec 1;328 ( Pt 2)(Pt 2):473-8. doi: 10.1042/bj3280473. Biochem J. 1997. PMID: 9371703 Free PMC article.
-
Cloning and expression of a cDNA for mu-class glutathione S-transferase from rabbit liver.Arch Biochem Biophys. 1995 Apr 20;318(2):424-9. doi: 10.1006/abbi.1995.1250. Arch Biochem Biophys. 1995. PMID: 7733673
-
De novo purine nucleotide biosynthesis: cloning, sequencing and expression of a chicken PurH cDNA encoding 5-aminoimidazole-4-carboxamide-ribonucleotide transformylase-IMP cyclohydrolase.Gene. 1991 Oct 15;106(2):197-205. doi: 10.1016/0378-1119(91)90199-l. Gene. 1991. PMID: 1937050
Cited by
-
A topologically conserved aliphatic residue in alpha-helix 6 stabilizes the hydrophobic core in domain II of glutathione transferases and is a structural determinant for the unfolding pathway.Biochem J. 1998 Dec 1;336 ( Pt 2)(Pt 2):413-8. doi: 10.1042/bj3360413. Biochem J. 1998. PMID: 9820819 Free PMC article.
-
Labeling of methyl groups: a streamlined protocol and guidance for the selection of 2H precursors based on molecular weight.J Biomol NMR. 2024 Sep;78(3):149-159. doi: 10.1007/s10858-024-00441-y. Epub 2024 May 24. J Biomol NMR. 2024. PMID: 38787508 Free PMC article.
-
Redesign of substrate-selectivity determining modules of glutathione transferase A1-1 installs high catalytic efficiency with toxic alkenal products of lipid peroxidation.Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9408-12. doi: 10.1073/pnas.150084897. Proc Natl Acad Sci U S A. 2000. PMID: 10900265 Free PMC article.
-
Phospholipid hydroperoxide glutathione peroxidase activity of human glutathione transferases.Biochem J. 1998 May 15;332 ( Pt 1)(Pt 1):97-100. doi: 10.1042/bj3320097. Biochem J. 1998. PMID: 9576856 Free PMC article.
-
Studies on the differential inhibition of glutathione conjugate formation of (+)-anti-benzo[a]pyrene 7,8-dihydrodiol 9,10-epoxide and 1-chloro-2,4-dinitrobenzene in V79 Chinese hamster cells.Biochem J. 2000 Aug 1;349 Pt 3(Pt 3):693-6. doi: 10.1042/bj3490693. Biochem J. 2000. PMID: 10903128 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Medical
Molecular Biology Databases