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. 1956 Mar 25;2(2):213-4.
doi: 10.1083/jcb.2.2.213.

A method for the histochemical differentiation of cholesterol and its esters

A method for the histochemical differentiation of cholesterol and its esters

I FEIGIN. J Biophys Biochem Cytol. .

Abstract

Free cholesterol is demonstrated in formalin-fixed frozen sections when treated successively by digitonin, alcohol-ether, and the Schultz technique, in which circumstances cholesterol esters are not visualized. Cholesterol esters and free cholesterol are both demonstrated in comparable sections treated by the Schultz method alone, so that the difference between such sections indicates the sites at which cholesterol esters may be considered present.

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