Trypsinization of monkey-kidney tissue: an automatic method for the preparation of cell suspensions

Abstract

A study of some of the factors which influenced the rate and amount of cells released from tissue fragments during trypsinization led to a revision of the method described by Youngner for monkey kidney. The revision includes the use of a glass mixing-chamber and magnetic stirrer in place of the Waring blendor. Simpler to use, the revised method has been found to yield, consistently, about 7 x 10 (7) cells per g of kidney tissue, or from two to three times more than that obtained by the earlier method.The revised method may be done either manually or automatically. A simple glass apparatus which automatically regulates the continuous addition of trypsin and removal of cell suspension during trypsinization has been developed. It operates reliably over a threefold volume range and a varying flow rate. The yield of cells per gram of tissue treated in the automatic trypsinizer is about 30% greater than when the change of fluids is done manually.