Adenosine triphosphate content of Mycobacterium leprae by percoll buoyant density centrifugation
Affiliations
- PMID: 1334044
Item in Clipboard
Adenosine triphosphate content of Mycobacterium leprae by percoll buoyant density centrifugation
Indian J Exp Biol.
1992 May.
Abstract
Thirty nine untreated patients of bacilliferous leprosy with a mean bacteriological index of 4.8 and morphological index of 1.3% formed the study group. Adenosine triphosphate assay was carried out by (i) enzyme treatment method in 18 patients and (ii) percoll buoyant density gradient method in 21 patients. ATP content obtained by percoll buoyant density gradient method was significantly higher than that obtained by enzyme treatment method. Percoll buoyant density centrifugation for purification and isolation of bacilli from human leproma is simplier, quicker and can serve as an alternate method of enzyme treatment.
Similar articles
-
Adenosine triphosphate content of Mycobacterium leprae isolated from armadillo tissue by Percoll buoyant density centrifugation.Int J Lepr Other Mycobact Dis. 1986 Mar;54(1):1-10. Int J Lepr Other Mycobact Dis. 1986. PMID: 3519794
-
Effect of chemotherapy on viability of Mycobacterium leprae as determined by ATP content, morphological index and FDA-EB fluorescent staining.Int J Lepr Other Mycobact Dis. 1989 Sep;57(3):615-21. Int J Lepr Other Mycobact Dis. 1989. PMID: 2476522
-
Treatment of bacilliferous BL/LL cases with combined chemotherapy and immunotherapy.Int J Lepr Other Mycobact Dis. 1995 Jun;63(2):202-12. Int J Lepr Other Mycobact Dis. 1995. PMID: 7602215
-
Acid-fast bacilli in semen; correlation with bacterial index.Int J Lepr Other Mycobact Dis. 1990 Sep;58(3):466-8. Int J Lepr Other Mycobact Dis. 1990. PMID: 2205682
-
Adenosine triphosphate content of Mycobacterium leprae from leprosy patients.Int J Lepr Other Mycobact Dis. 1984 Jun;52(2):183-8. Int J Lepr Other Mycobact Dis. 1984. PMID: 6373626