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. 1992 Dec 1;288 ( Pt 2)(Pt 2):637-42.
doi: 10.1042/bj2880637.

Purification and characterization of phosphatidylinositol 4-phosphate 5-kinases

N Divecha  1 , C E Brooksbank, R F Irvine
Affiliations

Purification and characterization of phosphatidylinositol 4-phosphate 5-kinases

N Divecha et al. Biochem J. .

Abstract

We detail the purification and characterization of three distinct isoforms of PtdIns4P 5-kinase present in bovine brain. One of these, PtdIns4P 5-kinase C, was purified to apparent homogeneity, and SDS/PAGE analysis demonstrated a single polypeptide and molecular mass 53 KDa. These three isoforms were shown to differ in their kinetic properties, and immunological characterization with an antibody raised to PtdIns4P 5-kinase C demonstrated that this isoform was unrelated to the other two. Furthermore, PtdIns4P 5-kinase C was shown to be the bovine brain homologue of the Type II PtdIns4P 5-kinase previously purified from human erythrocytes [Bazenet, Ruano, Brockman & Anderson (1990) J. Biol. Chem. 265, 18012-18022].

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References

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