Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1956 Oct 1;104(4):487-90.

Nephrotoxic serum nephritis in rats. II. Preparation and characterization of a soluble protective factor produced by trypsin digestion of rat tissue homogenates

J H BAXTER, H C GOODMAN

Nephrotoxic serum nephritis in rats. II. Preparation and characterization of a soluble protective factor produced by trypsin digestion of rat tissue homogenates

J H BAXTER et al. J Exp Med. .

Abstract

A soluble protective factor, capable of absorbing nephrotoxic antibodies from anti-rat kidney serum, can be obtained in high concentration by tryptic digestion of rat kidney homogenate. The factor is no longer antigenic or at most only slightly so. It is stable at 60 degrees , destroyed by boiling, is non-dialyzable, can be precipitated by ammonium sulfate, but resists destruction by proteolytic and other enzymes. In accord with previous studies on the organ localization of the rat tissue antigen responsible for the production of nephrotoxic antibodies, the soluble protective factor, or antigen derivative, can be obtained by tryptic digestion of rat lung, skeletal muscle, heart, and liver.

PubMed Disclaimer

References

    1. J Exp Med. 1956 Oct 1;104(4):467-85 - PubMed
    1. Proc Soc Exp Biol Med. 1951 Jul;77(3):498-501 - PubMed
    1. J Immunol. 1950 Jun;64(6):487-98 - PubMed
    1. J Biol Chem. 1948 Dec;176(3):1421-9 - PubMed
    1. J Exp Med. 1946 Aug 31;84(3):211-22 - PubMed