[The inhibitory effect of high energy shock waves on bladder tumor cell line BIU-87 in vitro]

Abstract

The cytotoxic effect of high energy shock waves (HESW) on bladder tumor cell line BIU-87 in vitro was evaluated by using the lithotripter Dornier HM3 with voltage 15 kV, impulsation 0, 400 and 800 respectively, water bath 35-37 degrees C and impulse rate 60/min. Cell viability and clonogenicity were decreased, but the cell doubling times were prolonged with the increasing number of HESW impulsation. Both cell growth curves and 3H-thymidine incorporation assay showed the temporary inhibition on the growth of tumor cells, which were viable after HESW. After a period of time, these tumor cells would recover their normal growth ability. For the group received 800 HESW impulses, the first passage of cell growth curve decreased temporarily 24 hours after HESW impulsation. These results indicate that HESW do show a cytotoxic effect on tumor cells in vitro, including acute and chronic lethal effect and temporary growth inhibition. The cytotoxic effect of HESW is positively proportional to the number of impulsation at the condition of definite working voltage.