Molecular cloning and chromosomal mapping of the human gene for the testis-specific catalytic subunit of calmodulin-dependent protein phosphatase (calcineurin A)
- PMID: 1339277
- DOI: 10.1016/0006-291x(92)92379-c
Molecular cloning and chromosomal mapping of the human gene for the testis-specific catalytic subunit of calmodulin-dependent protein phosphatase (calcineurin A)
Abstract
A cDNA for an alternatively spliced variant of the testis-specific catalytic subunit of calmodulin dependent protein phosphatase (CaM-PrP) was cloned from a human testis library. The nucleotide sequence of 2134 base pairs (bp) encodes a protein of 502 amino acids (Mr approximately 57,132) and pI 7.0. The cDNA sequence differs from the murine form of this gene by a 30 bp deletion in the coding region, the position of which matches those in the two other genes for the catalytic subunit. These data indicate that this alternative splicing event arose prior to the divergence of the three genes. The deduced sequence of the human protein is only 88% identical to the homologous murine form, in striking contrast to the other two CaM-PrP catalytic subunits which are highly conserved between mouse and human (approximately 99%); this indicates a more rapid rate of evolution for the testis-specific gene. Analysis of Southern blots containing DNA from human-hamster somatic cell hybrids show that the gene is on human chromosome 8.
Similar articles
-
Chromosomal mapping of the human genes for the calmodulin-dependent protein phosphatase (calcineurin) catalytic subunit.Biochem Biophys Res Commun. 1991 Nov 27;181(1):252-8. doi: 10.1016/s0006-291x(05)81410-x. Biochem Biophys Res Commun. 1991. PMID: 1659808
-
Molecular cloning of a calmodulin-dependent phosphatase from murine testis: identification of a developmentally expressed nonneural isoenzyme.Proc Natl Acad Sci U S A. 1992 Jan 15;89(2):529-33. doi: 10.1073/pnas.89.2.529. Proc Natl Acad Sci U S A. 1992. PMID: 1309945 Free PMC article.
-
Characterization of a cDNA clone encoding the calmodulin-binding domain of mouse brain calcineurin.Proc Natl Acad Sci U S A. 1988 Dec;85(23):8983-7. doi: 10.1073/pnas.85.23.8983. Proc Natl Acad Sci U S A. 1988. PMID: 2848250 Free PMC article.
-
Cloning and characterization of molecular isoforms of the catalytic subunit of calcineurin using nonisotopic methods.J Biol Chem. 1990 Jul 5;265(19):11312-9. J Biol Chem. 1990. PMID: 2162844
-
Isolation and sequence of rat testis cDNA for a calcium binding polypeptide similar to the regulatory subunit of calcineurin.Biochem Biophys Res Commun. 1991 Nov 14;180(3):1476-82. doi: 10.1016/s0006-291x(05)81362-2. Biochem Biophys Res Commun. 1991. PMID: 1659420
Cited by
-
Calcineurin regulates nuclear factor I dephosphorylation and activity in malignant glioma cell lines.J Biol Chem. 2013 Aug 16;288(33):24104-15. doi: 10.1074/jbc.M113.455832. Epub 2013 Jul 9. J Biol Chem. 2013. PMID: 23839947 Free PMC article.
-
Molecular analysis of CIB4 gene and protein in Kermani sheep.Braz J Med Biol Res. 2017 Sep 12;50(11):e6177. doi: 10.1590/1414-431X20176177. Braz J Med Biol Res. 2017. PMID: 28902924 Free PMC article.
-
A novel human protein serine/threonine phosphatase, which possesses four tetratricopeptide repeat motifs and localizes to the nucleus.EMBO J. 1994 Sep 15;13(18):4278-90. doi: 10.1002/j.1460-2075.1994.tb06748.x. EMBO J. 1994. PMID: 7925273 Free PMC article.
-
Immunological approach to identify calmodulin-stimulated phosphatase isozymes from bovine brain.Mol Cell Biochem. 1994 Mar 30;132(2):101-8. doi: 10.1007/BF00926918. Mol Cell Biochem. 1994. PMID: 7969092
-
Intragenic Locus in Human PIWIL2 Gene Shares Promoter and Enhancer Functions.PLoS One. 2016 Jun 1;11(6):e0156454. doi: 10.1371/journal.pone.0156454. eCollection 2016. PLoS One. 2016. PMID: 27248499 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous