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. 1992 Feb 28;1130(1):85-9.
doi: 10.1016/0167-4781(92)90467-e.

Cloning and characterization of the Dictyostelium discoideum rasG genomic sequences

S M Robbins  1 , J G Williams, G B Spiegelman, G Weeks
Affiliations

Cloning and characterization of the Dictyostelium discoideum rasG genomic sequences

S M Robbins et al. Biochim Biophys Acta. .

Abstract

A Dictyostelium discoideum genomic DNA clone containing the ras-related gene, rasG was isolated using the rasG cDNA as a probe. The genomic clone encompasses the entire coding region of the gene and 1.5 kb of 5' flanking region. The rasG gene contains a single intron as determined by sequence comparison with the cDNA, whereas the highly related rasD gene contains three introns. Primer extension analysis showed that transcription of the rasG gene initiates at multiple sites. Sequence analysis of the 5' flanking region of the gene revealed a stretch of thymine residues upstream from the transcription start sites but there is no evidence for a TATA box sequence.

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