[Cloning and gene expression of Bacillus cereus neutral proteinase in Bacillus subtilis cells]

Abstract

The neutral proteinase gene of Bacillus cereus was cloned. Its restriction map and the direction of transcription was determined. It was shown that the neutral proteinase gene could be expressed in Bacillus cells. The thermostability of the product coded by the neutral proteinase gene and its natural analogue was explored. The obtained data indicate that the neutral proteinase of Bacillus cereus is closely related to the enzyme of Bacillus amyloliquefaciens by these parameters. It was found that the neutral proteinase of Bacillus cereus has a high sensitivity to autolysis and that leads to the decrease in the enzymes concentration in the cultural medium at the late stages of cell growth. But the possibility of stabilization the neutral proteinase by Ca2+ ions has been demonstrated.