doi: 10.1007/BF01559385.
Functional role of soluble mitochondrial ATPase subunits
- PMID: 134032
- DOI: 10.1007/BF01559385
Item in Clipboard
Functional role of soluble mitochondrial ATPase subunits
J Bioenerg.
1976 Feb.
Abstract
A preparation of soluble mitochondrial ATPase (coupling factor F1) containing no gamma and delta minor subunits has been isolated. The minor-subunits-deficient F1 was found to be competent in ATP hydrolysis. However, it did not demonstrate a "coupling" effect in EDTA-submitochondrial particles. A portion of the ATPase activity of EDTA particles, stimulated by the minor-subunits-deficient F1, was insensitive to oligomycin. ATPase activity of Na+-particles was changed only slightly by this F1. It is suggested that gamma and delta subunits are necessary to form specific contacts between the F1 molecule and components of the mitochrondrial membrane.
Similar articles
-
Reconstitution of mitochondrial oligomycin and dicyclohexylcarbodiimide-sensitive ATPase.Eur J Biochem. 1980 Sep;110(1):225-35. doi: 10.1111/j.1432-1033.1980.tb04859.x. Eur J Biochem. 1980. PMID: 6108210
-
Action of alkyl cations and the natural ATPase inhibitor from mitochondria on soluble mitochondrial ATPase.Biochim Biophys Acta. 1977 Jul 7;461(1):101-8. doi: 10.1016/0005-2728(77)90072-x. Biochim Biophys Acta. 1977. PMID: 141940
-
The extent of mitochondrial F1-ATPase and adenine nucleotide carrier activity with epsilon-ATP.Biochim Biophys Acta. 1978 Feb 9;501(2):269-74. doi: 10.1016/0005-2728(78)90032-4. Biochim Biophys Acta. 1978. PMID: 145875
-
The structure of mitochondrial ATPase.Biochim Biophys Acta. 1973 Dec 31;301(3):249-77. doi: 10.1016/0304-4173(73)90006-2. Biochim Biophys Acta. 1973. PMID: 4273937 Review. No abstract available.
-
Recent developments on structural and functional aspects of the F1 sector of H+-linked ATPases.Mol Cell Biochem. 1984;60(1):33-71. doi: 10.1007/BF00226299. Mol Cell Biochem. 1984. PMID: 6231469 Review.
Cited by
-
Reconstitution of thermostable ATPase capable of energy coupling from its purified subunits.Proc Natl Acad Sci U S A. 1977 Mar;74(3):936-40. doi: 10.1073/pnas.74.3.936. Proc Natl Acad Sci U S A. 1977. PMID: 139610 Free PMC article.
-
Isolation of alpha-subunits of factor F1 from submitochondrial particles and the reconstitution of active ATPase from isolated alpha-subunits and beta-subunits bound to the mitochondrial membrane.Biochem J. 1980 Nov 15;192(2):483-8. doi: 10.1042/bj1920483. Biochem J. 1980. PMID: 6453586 Free PMC article.