Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Nov;2(3):180-5.
doi: 10.1038/ng1192-180.

Single pass sequencing and physical and genetic mapping of human brain cDNAs

A S Khan  1 , A S Wilcox, M H Polymeropoulos, J A Hopkins, T J Stevens, M Robinson, A K Orpana, J M Sikela
Affiliations

Single pass sequencing and physical and genetic mapping of human brain cDNAs

A S Khan et al. Nat Genet. 1992 Nov.

Abstract

We have performed single pass sequencing of 1,024 human brain cDNAs, over 900 of which seem to represent new human genes. Library prescreening with total brain cDNA significantly reduced repeated sequencing of highly represented cDNAs. A subset of sequenced cDNAs were physically mapped to their chromosomal locations using gene-specific STS primers derived from 3' untranslated regions. We have also determined that human brain cDNAs represent a rich source of gene-associated polymorphic markers. Microsatellite-containing cDNAs can be physically mapped and converted to highly informative genetic markers, thus facilitating integration of the human physical, expression and genetic maps.

PubMed Disclaimer

Comment in

  • Shake that 'body map'.
    [No authors listed] [No authors listed] Nat Genet. 1992 Nov;2(3):167-8. doi: 10.1038/ng1192-167. Nat Genet. 1992. PMID: 1345162 No abstract available.

Publication types

MeSH terms

LinkOut - more resources