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. 1992 Apr;45(4):324-7.
doi: 10.1136/jcp.45.4.324.

Restriction endonuclease analysis of Aspergillus fumigatus DNA

J P Burnie  1 , A Coke, R C Matthews
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Restriction endonuclease analysis of Aspergillus fumigatus DNA

J P Burnie et al. J Clin Pathol. 1992 Apr.

Abstract

Aims: To develop a genome based DNA fingerprinting system for Aspergillus fumigatus mould.

Methods: DNA was extracted from 21 isolates obtained from eight patients with an aspergilloma. This was with a freeze-dried mycelial extract fragmented in liquid nitrogen. DNA was subsequently purified by phenol-chloroform extraction followed by ultracentrifugation on a caesium chloride gradient. The DNA was restricted by EcoRI and Xba I.

Results: All isolates were identical when cut by EcoRI; Xba I delineated six DNA types.

Conclusions: DNA fingerprinting can be used to type isolates of A fumigatus. Strains from within an aspergilloma which were morphologically distinct could either have the identical DNA fingerprint or produce a unique type.

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