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. 1992 May 7;1131(1):108-10.
doi: 10.1016/0167-4781(92)90107-b.

Sequence and expression of rat ICAM-1

Y Kita  1 , T Takashi, Y Iigo, T Tamatani, M Miyasaka, T Horiuchi
Affiliations

Sequence and expression of rat ICAM-1

Y Kita et al. Biochim Biophys Acta. .

Abstract

We have isolated cDNA clones-coding for rat intercellular adhesion molecule-1 (RICAM-1) from a cDNA library constructed from rat Ax cells stimulated with IL-1 beta using the mouse ICAM-1 cDNA as a hybridization probe. The RICAM-1 sequence shows 79.1% homology with mouse ICAM-1 and 55.6% homology with human ICAM-1 at the nucleic acid level. In order to examine the expression of RICAM-1 on Chinese hamster ovary (CHO) cells, we constructed the vector, pSV-RICAM1-neo, containing the SV40 promoter. Flowcytometric analysis showed that CHO-K1 cells transfected with pSV-RICAM1-neo expressed high amounts of RICAM-1 on their surfaces.

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