The topography of tip growth in a plant cell

Abstract

Tips of young Phycomyces sporangiophores were dusted with starch grains, and growth photographically recorded. Rates of longitudinal displacement from the cell tip of individual markers were determined, also corresponding rates of change of cell diameter. From these the magnitude and spatial distribution of "relative elemental growth rates" along both longitudinal and circumferential axes of the cell were obtained. Growth rates in these two directions are functions of distance from the cell apex, and have different spatial distributions. In particular, rates of growth in cell circumference are complexly patterned. Relative elemental growth rates in length and in girth are approximately equal and maximal at the cell's apex, with a value of 2.4 mm. mm.(-1) hr.(-1). The characteristic shape of the tip is maintained constant in the face of its changing substance and position. This shape reflects a steady state of the cell's constituent growth patterns. At every point the growing membrane simultaneously expands in the two dimensions of its surface. The degree of polarization or directional preference of growth is measured by the ratio of longitudinal to circumferential relative elemental growth rate at any point. The ratio is not constant, but changes with position along the tip. This fact does not support the idea that membrane growth is based upon a quantal "growth event." Possible causal factors in oriented membrane growth are discussed.