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. 1992;24(4):228-33.
doi: 10.1159/000267172.

Simple and accurate assay for tyrosine hydroxylase from bovine retina by high-performance liquid chromatography with fluorescence detection

M Mandai  1 , M Iwaki, Y Honda
Affiliations

Simple and accurate assay for tyrosine hydroxylase from bovine retina by high-performance liquid chromatography with fluorescence detection

M Mandai et al. Ophthalmic Res. 1992.

Abstract

We present a simple and sensitive assay for tyrosine hydroxylase (TH) using high-performance liquid chromatography (HPLC) with a fluorescence detector which enabled us to study bovine retinal TH of a small quantity in crude retinal samples. The detection limit with this method was 1 pmol of dihydroxyphenylalanine (DOPA) enzymatically produced. Retinal TH exhibited the maximum activity at pH 6 to 6.3, and almost none at physiological pH of above 7, a tendency similar to that reported for striatal TH.

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