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. 1958 Nov 25;4(6):701-6.
doi: 10.1083/jcb.4.6.701.

The effect of HCl hydrolysis on the retention of thymidine in DNA

R E SAVAGE, W PLAUT

The effect of HCl hydrolysis on the retention of thymidine in DNA

R E SAVAGE et al. J Biophys Biochem Cytol. .

Abstract

Allium roots grown in C(14)-thymidine and H(3)-thymidine media were treated with N hydrochloric acid at 60 degrees C. as in standard Feulgen hydrolysis. The retention of the radioactive thymidine in DNA as a function of hydrolysis time was studied autoradiographically. No significant loss of label was detected until hydrolysis was extended beyond the optimal time for Feulgen staining. The data are consistent with the assumption that there is no significant loss of DNA during normal Feulgen hydrolysis in the material used.

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References

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    1. J Biophys Biochem Cytol. 1957 Jan 25;3(1):71-88 - PubMed