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. 1991 Mar;102(3):581-4.
doi: 10.1111/j.1476-5381.1991.tb12216.x.

Selective and competitive antagonism by suramin of ATP-stimulated catecholamine-secretion from PC12 phaeochromocytoma cells

K Inoue  1 , K Nakazawa, M Ohara-Imaizumi, T Obama, K Fujimori, A Takanaka
Affiliations

Selective and competitive antagonism by suramin of ATP-stimulated catecholamine-secretion from PC12 phaeochromocytoma cells

K Inoue et al. Br J Pharmacol. 1991 Mar.

Abstract

1. Suramin, a putative P2-antagonist, (10 to 300 microM) inhibited the adenosine 5'-triphosphate (ATP)-stimulated secretion of [3H]-noradrenaline or endogenous dopamine from phaeochromocytoma PC12 cells in a concentration-dependent manner. Suramin (300 microM) did not affect the dopamine-secretion stimulated by high K+ or nicotine. 2. Suramin shifted the concentration-response curve for ATP to the right. The antagonism was competitive with a pA2 value of 4.52. 3. ATP also stimulated an increase in intracellular Ca2+ concentration as determined by fura-2 methods. Suramin antagonized this effect over the same concentration range that antagonized the ATP-stimulated catecholamine secretion. 4. These results suggest that suramin can be used as a selective and competitive antagonist of ATP in experiments concerning mechanisms of catecholamine-secretion.

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References

    1. Ion Channels. 1990;2:169-203 - PubMed
    1. Br J Pharmacol. 1990 Sep;101(1):224-6 - PubMed
    1. Eur J Pharmacol. 1989 Aug 3;166(3):531-4 - PubMed
    1. Circ Res. 1986 Mar;58(3):319-30 - PubMed
    1. J Biol Chem. 1985 Mar 25;260(6):3440-50 - PubMed

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