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. 1976 Oct;3(10):2665-75.
doi: 10.1093/nar/3.10.2665.

Studies on the biological role of DNA methylation. II. Role of phiX174 DNA methylation in the process of viral progeny DNA synthesis

J Friedman, A Razin
Free PMC article

Studies on the biological role of DNA methylation. II. Role of phiX174 DNA methylation in the process of viral progeny DNA synthesis

J Friedman et al. Nucleic Acids Res. 1976 Oct.
Free PMC article

Abstract

In vivo inhibition of bacteriophage phiX174 DNA methylation by nicotinamide resulted in the accumulation of replicative intermediates with multiple-genome length single-stranded "tails". These abnormal replicative intermediates could not be chased into viral single-stranded circular DNA. The effect of nicotinamide on phage maturation and accumulation of abnormal replicative intermediates could be reversed by washing out the inhibitor. The results suggest that the single methyl group present in the viral DNA serves as a recognition site for a specific endonuclease, probably the gene A protein product, that is responsible for the excision of the single-stranded one-genome long viral DNA, before final maturation of the virus occurs.

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References

    1. Nucleic Acids Res. 1975 Oct;2(10):1967-74 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Feb;69(2):475-9 - PubMed
    1. J Virol. 1972 Sep;10(3):384-91 - PubMed
    1. J Mol Biol. 1973 Aug 15;78(3):417-25 - PubMed
    1. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3773-5 - PubMed

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