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. 1991 Jan;9(1):47-52.
doi: 10.1038/nbt0191-47.

Production of human interleukin-3 using industrial microorganisms

R W van Leen  1 , J G Bakhuis, R F van Beckhoven, H Burger, L C Dorssers, R W Hommes, P J Lemson, B Noordam, N L Persoon, G Wagemaker
Affiliations

Production of human interleukin-3 using industrial microorganisms

R W van Leen et al. Biotechnology (N Y). 1991 Jan.

Abstract

We expressed a cDNA encoding the multicolony stimulating factor interleukin-3 in a variety of cell types, including bacteria, yeast and mammalian cells. After evaluation of the advantages and disadvantages of each potential system, we designed a production and purification scheme using Bacillus licheniformis. The purification consists of hydrophobic interaction chromatography, two steps of ion exchange chromatography and gel filtration. The purified and formulated product entered clinical trials in November 1989.

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