Molecular cloning and characterization of the recA gene of Methylomonas clara and construction of recA deficient mutant
- PMID: 1367275
- DOI: 10.1007/BF00180631
Molecular cloning and characterization of the recA gene of Methylomonas clara and construction of recA deficient mutant
Abstract
The recA gene of the methylotrophic bacterium Methylomonas clara has been isolated from a genomic library by hybridization with the Escherichia coli recA gene. Its complete nucleotide sequence consists of 1029 bp encoding a polypeptide of 342 amino acids. Nucleotide sequence analysis of the M. clara recA gene revealed extensive homologies to recA genes from E. coli and Pseudomonas aeruginosa. Part of the physiological activity of the M. clara RecA protein has become evident in that E. coli recA mutant HB101 is complemented. The cloned recA gene has been modified in vitro by site-specific mutagenesis and by insertion of a kanamycin-resistance gene cassette into the recA coding sequence. M. clara recA mutants were obtained by replacement of the active recA gene by an in-vitro inactivated gene copy.
Similar articles
-
Cloning and sequencing the recA+ genes of Acetobacter polyoxogenes and Acetobacter aceti: construction of recA- mutants of by transformation-mediated gene replacement.Gene. 1993 May 15;127(1):47-52. doi: 10.1016/0378-1119(93)90615-a. Gene. 1993. PMID: 8486287
-
Cloning, sequence and expression in Escherichia coli of the Methylobacillus flagellatum recA gene.Gene. 1990 Sep 28;94(1):69-75. doi: 10.1016/0378-1119(90)90469-8. Gene. 1990. PMID: 2227454
-
Molecular cloning, sequence and regulation of expression of the recA gene of the phototrophic bacterium Rhodobacter sphaeroides.Mol Gen Genet. 1994 Jan;242(1):116-20. doi: 10.1007/BF00277356. Mol Gen Genet. 1994. PMID: 8277942
-
Analysis of the DNA binding site of Escherichia coli RecA protein.Adv Biophys. 1995;31:23-48. doi: 10.1016/0065-227x(95)99381-x. Adv Biophys. 1995. PMID: 7625276 Review.
-
Molecular genetics of methylotrophic bacteria.Biotechnology. 1991;18:273-304. doi: 10.1016/b978-0-7506-9188-8.50019-x. Biotechnology. 1991. PMID: 1909917 Review. No abstract available.
Cited by
-
A deletion polymorphism in the human alpha-2-macroglobulin (A2M) gene.Nucleic Acids Res. 1991 Sep 25;19(18):5102. doi: 10.1093/nar/19.18.5102. Nucleic Acids Res. 1991. PMID: 1717945 Free PMC article.
-
Isolation of the Helicobacter pylori recA gene and involvement of the recA region in resistance to low pH.Infect Immun. 1995 Jun;63(6):2185-93. doi: 10.1128/iai.63.6.2185-2193.1995. Infect Immun. 1995. PMID: 7768597 Free PMC article.
-
New nucleotide sequence data on the EMBL File Server.Nucleic Acids Res. 1991 Sep 25;19(18):5103-10. doi: 10.1093/nar/19.18.5103. Nucleic Acids Res. 1991. PMID: 1923795 Free PMC article. No abstract available.
-
Interspecies regulation of the recA gene of gram-negative bacteria lacking an E. coli-like SOS operator.Mol Gen Genet. 1994 Nov 15;245(4):523-7. doi: 10.1007/BF00302266. Mol Gen Genet. 1994. PMID: 7808403
-
The RecA protein as a model molecule for molecular systematic studies of bacteria: comparison of trees of RecAs and 16S rRNAs from the same species.J Mol Evol. 1995 Dec;41(6):1105-23. doi: 10.1007/BF00173192. J Mol Evol. 1995. PMID: 8587109 Free PMC article.