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. 1991 Sep;9(9):844-7.
doi: 10.1038/nbt0991-844.

Generation of transgenic dairy cattle using 'in vitro' embryo production

P Krimpenfort  1 , A Rademakers, W Eyestone, A van der Schans, S van den Broek, P Kooiman, E Kootwijk, G Platenburg, F Pieper, R Strijker, et al.
Affiliations

Generation of transgenic dairy cattle using 'in vitro' embryo production

P Krimpenfort et al. Biotechnology (N Y). 1991 Sep.

Abstract

We have combined gene transfer, by microinjection, with 'in vitro' embryo production technology, enabling us to carry out non-surgical transfer, to recipient cows, of microinjected embryos that have been cultured from immature oocytes. Using this approach, we have established 21 pregnancies from which 19 calves were born. Southern blot analysis proved that in two cases the microinjected DNA had been integrated in the host genome.

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