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. 1990 Jun;33(3):287-90.
doi: 10.1007/BF00164523.

Anchorage-dependent mammalian cell culture using polyurethane foam as a new substratum for cell attachment

T Matsushita  1 , M Ketayama, K Kamihata, K Funatsu
Affiliations

Anchorage-dependent mammalian cell culture using polyurethane foam as a new substratum for cell attachment

T Matsushita et al. Appl Microbiol Biotechnol. 1990 Jun.

Abstract

Anchorage-dependent mammalian cells were cultivated at high cell density in a novel culture system using polyurethane foam (PUF) as a substratum for cell attachment. PUF has a macroporous structure giving a high surface area to volume ratio. Monkey kidney cells (Vero) and Chinese hamster ovary cells (CHO-K1) attached to the internal surface of PUF and grew to a high cell density (1.04 X 10(8) cells/cm3 PUF and 3.5 X 10(7) cells/cm3 PUF, respectively) in PUF stationary cultures. In addition, we have designed a PUF-particle packed-bed culture system for high density mass cell culture. A maximum cell density of 2.4 X 10(7) cells/cm3 culture vessel volume was obtained in a packed-bed culture of Vero cells.

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