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. 1991 Sep;9(9):835-8.
doi: 10.1038/nbt0991-835.

Transgenic production of a variant of human tissue-type plasminogen activator in goat milk: generation of transgenic goats and analysis of expression

K M Ebert  1 , J P Selgrath, P DiTullio, J Denman, T E Smith, M A Memon, J E Schindler, G M Monastersky, J A Vitale, K Gordon
Affiliations

Transgenic production of a variant of human tissue-type plasminogen activator in goat milk: generation of transgenic goats and analysis of expression

K M Ebert et al. Biotechnology (N Y). 1991 Sep.

Abstract

We report the first successful production of transgenic goats that express a heterologous protein in their milk. The production of a glycosylation variant of human tPA (LAtPA--longer acting tissue plasminogen activator) from an expression vector containing the murine whey acid promoter (WAP) operatively linked to the cDNA of a modified version of human tPA was examined in transgenic dairy goats. Two transgenic goats were identified from 29 animals born. The first animal, a female, was mated and allowed to carry the pregnancy to term. Milk was obtained upon parturition and was shown to contain enzymatically active LAtPA at a concentration of 3 micrograms/ml.

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