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. 1959 Aug 14;130(3372):386-7.
doi: 10.1126/science.130.3372.386.

Use of phosphorus-32 in microassay for nucleic acid synthesis in Escherichia coli

P HANAWALT

Use of phosphorus-32 in microassay for nucleic acid synthesis in Escherichia coli

P HANAWALT. Science. .

Abstract

A method was developed to facilitate studies of ribonucleic acid and deoxyribonucleic acid synthesis in growing bacterial cultures of small volume. The nucleic acid fractions were separated by means of a modified Schmidt-Thannhauser procedure applied to micro quantities of P(32)-labeled cells collected on membrane filters. The validity and usefulness of the method are discussed.

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