Application of statistical design of experiments to the optimization of factor VIII expression by CHO cells

Abstract

Selection and optimization of the concentrations of chemically defined components effective on recombinant factor VIII:C production by CHO cells have been performed with statistically designed experiments. Screening of efficient compounds on factor VIII expression was performed by using HADAMARD method, while a precise optimization of the concentrations of two components was achieved with the help of DOEHLERT method. Increased specific activity of factor VIII and reduced cost medium have been obtained with a fewer number of experiments than compared to a less rational approach.