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Comparative Study
. 1992 Jan;36(4):503-6.
doi: 10.1007/BF00170192.

In vitro mutagenesis of a xylanase from the extreme thermophile Caldocellum saccharolyticum

E Lüthi  1 , K Reif, N B Jasmat, P L Bergquist
Affiliations
Comparative Study

In vitro mutagenesis of a xylanase from the extreme thermophile Caldocellum saccharolyticum

E Lüthi et al. Appl Microbiol Biotechnol. 1992 Jan.

Abstract

Six mutant xylanases were obtained by in vitro mutagenesis of a xylanase gene from the extremely thermophilic bacterium Caldocellum saccharolyticum. The temperature stability of all enzymes was affected by mutation to various degrees and one of the xylanases had an altered temperature optimum. The mutations had no effect on the pH optimum. The C. saccharolyticum xylanase showed strong homology to several thermophilic and mesophilic xylanases, and comparison of primary sequences allowed the localization of probable active sites and residues involved in thermostability.

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