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. 1991 Dec;55(12):3053-7.

Transformation of Penicillium urticae with plasmids containing the hygromycin B resistance gene

N Kiuchi  1 , A Naruse, H Yamamoto, J Sekiguchi
Affiliations
  • PMID: 1368765
Free article

Transformation of Penicillium urticae with plasmids containing the hygromycin B resistance gene

N Kiuchi et al. Agric Biol Chem. 1991 Dec.
Free article

Abstract

A transformation system with efficiencies between 6 and 50 stable transformants per micrograms of DNA was developed for Penicillium urticae J1 (ATCC48163) using hygromycin B-resistant plasmids containing or not containing fragments of the P. urticae genome. The tandem repeated integration and/or random integration of vector DNA were observed. Although P. urticae was unable to grow in the presence of 200 micrograms/ml hygromycin B, the transformants were resistant to more than 5 mg/ml of hygromycin B.

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