Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Jan;3(1):31-7.
doi: 10.1007/BF00365263.

A human-human hybridoma secreting anti-Pseudomonas aeruginosa exotoxin-A monoclonal antibody with highly potent neutralizing activity

M Kuriyama  1 , Y Ichimori, S Iwasa, K Tsukamoto
Affiliations

A human-human hybridoma secreting anti-Pseudomonas aeruginosa exotoxin-A monoclonal antibody with highly potent neutralizing activity

M Kuriyama et al. Cytotechnology. 1990 Jan.

Abstract

A hybridoma secreting human monoclonal antibody (MAB) against Pseudomonas aeruginosa exotoxin A (PEA) was constructed by fusing Epstein-Barr virus-transformed peripheral blood lymphocytes with human B lymphoblastoid cell line TAW-925. The human-human hybridoma stably produced human IgG2 MAB at the rate of 0.4-0.5 microgram/ml per 10(6) cells per day for more than six months, and the MAB was capable of neutralizing the in vitro cytotoxic and in vivo lethal effects of PEA with approximately 100- and 70-fold, respectively, higher activity than serum polyclonal antibody preparations.

PubMed Disclaimer

References

    1. Nature. 1980 Dec 4;288(5790):488-9 - PubMed
    1. J Immunol. 1981 Oct;127(4):1275-80 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5429-31 - PubMed
    1. J Immunol. 1983 Jan;130(1):165-8 - PubMed
    1. J Gen Microbiol. 1987 Dec;133(12):3581-90 - PubMed

Publication types

MeSH terms

LinkOut - more resources