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. 1991 Jul;13(1):21-4.
doi: 10.1111/j.1472-765x.1991.tb00559.x.

DNA recovery and direct detection of Tn5 sequences from soil

S Selenska  1 , W Klingmüller
Affiliations

DNA recovery and direct detection of Tn5 sequences from soil

S Selenska et al. Lett Appl Microbiol. 1991 Jul.

Abstract

Specific Tn5 sequences inserted in the genome of Enterobacter agglomerans were detected in EcoRI digested DNA directly recovered from soil 70 d after its inoculation with the bacteria, when these were no longer culturable on agar medium. A new method of DNA extraction from soil was used. No amplification of DNA sequences by PCR was needed.

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