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Review
. 1992 Oct;3(5):523-32.
doi: 10.1016/0958-1669(92)90081-s.

Examples of expression systems based on animal RNA viruses: alphaviruses and influenza virus

C M Rice  1
Affiliations
Review

Examples of expression systems based on animal RNA viruses: alphaviruses and influenza virus

C M Rice. Curr Opin Biotechnol. 1992 Oct.

Abstract

Successful recovery of RNA viruses and functional RNA replicons from cDNA has greatly facilitated molecular genetic analyses of viral proteins and cis-regulatory elements. This technology allows the use of RNA virus replication machinery to express heterologous sequences. Both positive-strand and negative-strand animal RNA viruses have been engineered to produce chimeric viruses expressing protective epitopes from other pathogens and for transient expression of heterologous sequences.

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References

    1. Bredenbeek PJ, Rice CM. Animal RNA Virus Expression Systems. Sem Virol. 1992;3:297–310.
    1. Ahlquist P, French R, Janda M, Loesch-Fries LS. Vol. 81. 1984. Multi-component RNA Plant Virus Infection Derived from Cloned Viral cDNA; pp. 7066–7070. (Proc Natl Acad Sci USA). - PMC - PubMed
    1. Ball LA. Cellular Expression of a Functional Nodavirus RNA Replicon from Vaccinia Virus Vectors. J Virol. 1992;66:2335–2345. - PMC - PubMed
    1. Pattnaik AK, Ball LA, Legrone AW, Wertz GW. Infectious Defective Interfering Particles of VSV from Transcripts of a cDNA Clone. Cell. 1992;69:1011–1020. of special interest. - PubMed

    2. This report describes the first successful recovery of a replication-competent rhabdovirus RNA transcript from cDNA and the expression of heterologous RNA sequences using this viral replication machinery.

    1. Almond JW, Burke KL. Poliovirus as a Vector for the Presentation of Foreign Antigens. Semin Virol. 1990;1:11–20.

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