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. 1960 Dec 1;112(6):1195-210.
doi: 10.1084/jem.112.6.1195.

The preparation and some properties of purified antibody specific for the 2,4-dinitrophenyl group

F S FARAH, M KERN, H N EISEN

The preparation and some properties of purified antibody specific for the 2,4-dinitrophenyl group

F S FARAH et al. J Exp Med. .

Abstract

Injection of rabbits with Freund's complete adjuvant containing bovine gamma-globulin substituted to near saturation with dinitrophenyl groups leads to the production of large amounts of antibodies specific for the 2,4-dinitrophenyl substituent. These antibodies can be isolated from sera in satisfactory yield (40 per cent) and in a high state of purity (90 per cent specifically precipitable). Hapten dissolution, which was used to screen haptens for their potential usefulness as specific reagents in the purification procedure, provides a means for evaluating, in relative terms, antibody-hapten affinities (in competition with antigen).

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References

    1. J Exp Med. 1959 Aug 1;110(2):187-206 - PubMed
    1. J Exp Med. 1936 Jul 31;64(2):161-72 - PubMed
    1. J Immunol. 1956 Sep;77(3):193-7 - PubMed
    1. J Exp Med. 1960 Dec 1;112:1227-47 - PubMed
    1. J Immunol. 1954 Nov;73(5):296-308 - PubMed