Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1990 Sep;4(2):163-71.
doi: 10.1007/BF00365097.

Production of recombinant Von Willebrand factor by CHO cells cultured in macroporous microcarriers

G Mignot  1 , T Faure, V Ganne, B Arbeille, A Pavirani, J L Romet-Lemonne
Affiliations
Comparative Study

Production of recombinant Von Willebrand factor by CHO cells cultured in macroporous microcarriers

G Mignot et al. Cytotechnology. 1990 Sep.

Abstract

Recombinant Chinese hamster ovary cells producing Von Willebrand factor have been successfully grown in gelatin macroporous microcarriers (Cultispher-G). Serum-free cultures were maintained in 1, 4, and 10 liter fermentors for more than two months. Comparative studies with Cytodex-3 microcarriers have been performed in 1 liter fermentors. The lower specific Von Willebrand factor productivity of CHO cells cultivated on Cultispher-G were offset by higher cell densities (10(7) - 2 x 10(7) cells/ml). Volumetric Von Willebrand factor productivity was influenced by oxygen concentration, and remained stable during scale-up from 1 to 10 liter fermentors.

PubMed Disclaimer

References

    1. J Cell Sci. 1983 May;61:351-63 - PubMed
    1. Rev Infect Dis. 1984 May-Jun;6 Suppl 2:S341-4 - PubMed
    1. Cytotechnology. 1988 Nov;1(4):339-46 - PubMed
    1. Nature. 1967 Oct 7;216(5110):64-5 - PubMed
    1. Dev Biol Stand. 1981;50:103-10 - PubMed

Publication types

LinkOut - more resources