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. 1992 Jan 31;182(2):851-7.
doi: 10.1016/0006-291x(92)91810-d.

Expression of guanylate cyclase-A mRNA in the rat retina: detection using polymerase chain reaction

R K Kutty  1 , R T Fletcher, G J Chader, G Krishna
Affiliations

Expression of guanylate cyclase-A mRNA in the rat retina: detection using polymerase chain reaction

R K Kutty et al. Biochem Biophys Res Commun. .

Abstract

A technique based on RNA-PCR was successfully employed for the detection of guanylate cyclase-A (GC-A) mRNA in the rat retina. Three sets of primers designed from the published cDNA sequence of rat brain guanylate cyclase-A (GC-A) produced amplification products of expected sizes from the retina as well as brain. Analysis of retinal PCR products yielded a 970 bp sequence, which showed 100% homology to the cDNA sequence of GC-A (2343-3312 bp region). Northern blot analysis was not very sensitive for the detection of GC-A mRNA in the retina. The results indicate that the mRNA for GC-A (or a closely related form) is probably expressed in the retina, but at a lower level than that found in the brain.

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