Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Jan 25;20(2):171-8.
doi: 10.1093/nar/20.2.171.

Methylation of an alpha-foetoprotein gene intragenic site modulates gene activity

Affiliations
Free PMC article

Methylation of an alpha-foetoprotein gene intragenic site modulates gene activity

K Opdecamp et al. Nucleic Acids Res. .
Free PMC article

Abstract

By comparing the methylation pattern of Mspl/Hpall sites in the 5' region of the mouse alpha-foetoprotein (AFP) gene of different cells (hepatoma cells, foetal and adult liver, fibroblasts), we found a correlation between gene expression and unmethylation of a site located in the first intron of the gene. Other sites did not show this correlation. In transfection experiments of unmethylated and methylated AFP-CAT chimeric constructions, we then showed that methylation of the intronic site negatively modulates expression of CAT activity. We also found that a DNA segment centered on this site binds nuclear proteins; however methylation did not affect protein binding.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Genes Dev. 1989 May;3(5):612-9 - PubMed
    1. Mol Cell Biol. 1989 Apr;9(4):1415-25 - PubMed
    1. Virology. 1988 Sep;166(1):166-74 - PubMed
    1. Cell. 1987 Nov 6;51(3):435-43 - PubMed
    1. Mol Cell Biol. 1985 Nov;5(11):2984-92 - PubMed

Publication types