Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Feb 28;183(1):163-9.
doi: 10.1016/0006-291x(92)91623-x.

Cloning of murine and rat vascular cell adhesion molecule-1

C Hession  1 , P Moy, R Tizard, P Chisholm, C Williams, M Wysk, L Burkly, K Miyake, P Kincade, R Lobb
Affiliations

Cloning of murine and rat vascular cell adhesion molecule-1

C Hession et al. Biochem Biophys Res Commun. .

Abstract

Vascular cell adhesion molecule-1 (VCAM1) is a member of the immunoglobulin (Ig) superfamily which interacts with the integrin very late antigen 4 (VLA4). We have cloned the cDNAs for both murine and rat VCAM1 from endotoxin-treated lung libraries. Both sequences encode proteins with seven extracellular Ig-like domains, which show 75.9% and 76.9% identity, respectively, with human VCAM1. Both murine and human cell lines show VLA4-dependent binding to COS cells transiently expressing murine and rat VCAM1. Two mAbs, M-K/1 and M-K/2, which recognize an antigen on murine bone marrow stromal cell lines, bind to murine VCAM1 expressed in COS cells and block VCAM1-dependent adhesion, confirming that these mAbs recognize murine VCAM1.

PubMed Disclaimer

Publication types

Substances

LinkOut - more resources