Comparative Study
doi: 10.1016/0006-291x(92)91634-3.
Purification of nitric oxide synthase from bovine brain: immunological characterization and tissue distribution
Affiliations
- PMID: 1371919
- DOI: 10.1016/0006-291x(92)91634-3
Item in Clipboard
Comparative Study
Purification of nitric oxide synthase from bovine brain: immunological characterization and tissue distribution
Biochem Biophys Res Commun.
.
Abstract
Nitric oxide (NO) synthase (EC 1.14.23) was purified to homogeneity from bovine cerebrum. The molecular weight of NO synthase was estimated to be 150 kDa by both SDS/PAGE and gel filtration at high salt concentration. For activity, the enzyme required NADPH, Ca2+, calmodulin and tetrahydrobiopterin as cofactors. Rabbit polyclonal antibody to bovine brain NO synthase reacted with 150 kDa NO synthase in various bovine and rat organs, including the brain, pituitary and adrenal glands, but not with that in stimulated macrophages, indicating that there are at least two immunologically distinct NO synthases.
Similar articles
-
Purification of a distinctive form of endotoxin-induced nitric oxide synthase from rat liver.Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5361-5. doi: 10.1073/pnas.89.12.5361. Proc Natl Acad Sci U S A. 1992. PMID: 1376917 Free PMC article.
-
Ca2+/calmodulin-dependent formation of hydrogen peroxide by brain nitric oxide synthase.Biochem J. 1992 Feb 1;281 ( Pt 3)(Pt 3):627-30. doi: 10.1042/bj2810627. Biochem J. 1992. PMID: 1371384 Free PMC article.
-
A bacterial nitric oxide synthase from a Nocardia species.Biochem Biophys Res Commun. 1994 Sep 15;203(2):1251-8. doi: 10.1006/bbrc.1994.2317. Biochem Biophys Res Commun. 1994. PMID: 7522444
-
Approaches toward selective inhibition of nitric oxide synthase.J Med Chem. 1994 Jun 24;37(13):1899-907. doi: 10.1021/jm00039a001. J Med Chem. 1994. PMID: 7518001 Review. No abstract available.
-
Isoforms of nitric oxide synthase. Characterization and purification from different cell types.Biochem Pharmacol. 1991 Oct 24;42(10):1849-57. doi: 10.1016/0006-2952(91)90581-o. Biochem Pharmacol. 1991. PMID: 1720618 Review. No abstract available.
Cited by
-
[Determination of NO-synthase activity in the brain (new method)].Biull Eksp Biol Med. 1994 Jan;117(1):39-41. doi: 10.1007/BF02444075. Biull Eksp Biol Med. 1994. PMID: 7514899 Russian. No abstract available.
-
Pathological characterization of astrocytic hyaline inclusions in familial amyotrophic lateral sclerosis.Am J Pathol. 1997 Aug;151(2):611-20. Am J Pathol. 1997. PMID: 9273821 Free PMC article.
-
Purification and characterization of nitric oxide synthase (NOSNoc) from a Nocardia species.J Bacteriol. 1995 Sep;177(17):5122-8. doi: 10.1128/jb.177.17.5122-5128.1995. J Bacteriol. 1995. PMID: 7545152 Free PMC article.
-
Nitric oxide synthase isozymes antibodies.Histochem J. 1995 Oct;27(10):738-44. Histochem J. 1995. PMID: 8575937 Review.
-
Nitric oxide synthase in ferret brain: localization and characterization.Br J Pharmacol. 1992 Nov;107(3):849-52. doi: 10.1111/j.1476-5381.1992.tb14535.x. Br J Pharmacol. 1992. PMID: 1282076 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
