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. 1960 Dec 7:35:745-51.

Blood level of indocyanine green in the dog during multiple dye curves and its effect on instrumental calibration

A W EDWARDS, J B BASSINGTHWAIGHTE, W F SUTTERER, E H WOOD

Blood level of indocyanine green in the dog during multiple dye curves and its effect on instrumental calibration

A W EDWARDS et al. Proc Staff Meet Mayo Clin. .
No abstract available

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Figures

Fig. 1
Fig. 1
Concentration of indocyanine green in blood of dog after single and multiple injections of dye. Broken lines represent exponential decrease in concentration. Concentration of dye in plasma x (1-hematocrit reading) is used as the concentration in blood.
Fig. 2
Fig. 2
Concentration of indocyanine in blood of two dogs after multiple injections of dye. Broken lines represent exponential decrease in concentration. In dog represented in lower panel, with smaller injections of dye, level of background dye remained low and was approximately the same at end of each series of injections as it was at the end of the preceding series.
Fig. 3
Fig. 3
Calibration curves for increments in concentration of indocyanine green in blood in presence of background dye. Upper panels show calibration curves in densitometers D1 and D2U respectively when the galvanometer zero positions were set to coincide with the reading obtained with zero light on the phototube, and the sensitivity of the instruments adjusted to give equal full-scale deflections on the “blank” blood sample pertaining to the respective levels of background dye. In D1 presence of 10.9 mg. of background dye per liter distorts curve so that use of original calibration curve would give values for increments in dye concentration that would be 11.2 per cent too small. A leak of light around filter in D2U produced gross differences in calibration at various levels of background dye. Lower panels illustrate excellent compensation achieved by introduction of zero-suppression circuit which causes galvanometer reading with zero light on the phototube to deflect in negative direction from galvanometer zero. Last triangle in lower right panel represents total (background and increment dye) concentration of 60 mg. per liter.
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References

    1. Dow Philip. Estimations of Cardiac Output and Central Blood Volume by Dye Dilution. Physiol Rev. 1956 Jan;36:77–102. - PubMed
    1. Edwards AWT, Korner PI. Factors Determining the Dispersion of Dye in Indicator Dilution Curves in the Normal Mammalian Circulation. Clin Sc. 1958 May;17:265–278. - PubMed
    1. Fox IJ, Brooker LGS, Heseltine DW, Essex HE, Wood EH. A Tricarbocyanine Dye for the Continuous Recording of Dilution Curves in Whole Blood Independent of Variations in Blood Oxygen Saturation. Proc Staff Meet, Mayo Clin. 1957 Sept 4;32:478–484. - PubMed
    1. Wheeler HO, Cranston WI, Meltzer JI. Hepatic Uptake and Biliary Excretion of Indocyanine Green in the Dog. Proc Soc Exper Biol & Med. 1958 Oct;99:11–14. - PubMed
    1. Edwards AWT, Isaacson J, Sutterer WF, Bassingthwaighte JB, Wood EH. An Interference Filter Densitometer and Its Use For Measurement of Indocyanine Green in Flowing Blood. (Unpublished data.)

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